Accession Number:

ADA499711

Title:

Integrated Semiconductor-based Diagnostics System for Multiplexed Genomic Amplification and Electrochemical Detection of Biothreat Agents

Descriptive Note:

Final rept. 1 Aug 2006-31 Jul 2008

Corporate Author:

COMBIMATRIX CORP MUKILTEO WA

Personal Author(s):

Report Date:

2009-01-01

Pagination or Media Count:

85.0

Abstract:

This contract effort focused on the development of an automated, cartridge-based genotyping system that integrates a multiplex polymerase chain reaction PCR assay with a semiconductor microarray that uses electrochemical detection to identify biothreat agents. The DX-100 Genotyping Cartridge System has three subcomponents a disposable cartridge, the cartridge processing instrument, and a personal computer. The alpha prototype is compact and solid state no optics and has a simple three step operation 1. Load the cartridge with a DNA sample 2. Load the cartridge in the instrument 3. Move the operating handle to the front. A personal computer with integrated software controls the instruments operation, collects electrical readings from each electrode on the microarray, analyses the data, and provides a graphic output for agent identification. The fluidic cartridge is sealed and contains all of the reagents and a microarray to test for Bacillus anthracis, Clostridium botulinum, Yersinia pestis, Francisella tularensis, Burkholderia pseudomallei, Brucella suis, Vibrio cholera, and Yersinia enterocolitica.

Subject Categories:

  • Physical Chemistry
  • Chemical, Biological and Radiological Warfare
  • Miscellaneous Detection and Detectors
  • Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE