Accession Number:

ADA499664

Title:

Glutamate Receptor Aptamers and ALS

Descriptive Note:

Annual rept. 8 Dec 2007-7 Dec 2008

Corporate Author:

STATE UNIV OF NEW YORK AT ALBANY RESEARCH FOUNDATION

Personal Author(s):

Report Date:

2009-01-01

Pagination or Media Count:

30.0

Abstract:

Excitotoxicity is one of the leading causes for amyotrophic lateral sclerosis ALS. Our goal was to develop a novel class of powerful aptamer-based, anti-excitotoxic inhibitors against GluR2Qflip, a key AMPA receptor subunit that controls the calcium permeability and mediates excitotoxicity. An aptamer is a single-stranded nucleic acid that directly inhibits a proteins function by folding into a specific tertiary structure that dictates high-affinity binding to the target protein. To date, we have identified two classes of aptamers i.e. competitive and noncompetitive aptamers against GluR2Qflip, by using a molecular biology approach called systematic evolution of ligands by exponential enrichment SELEX. These aptamers are water soluble and have a nanomolar affinity against GluR2Qflip. Their inhibitory properties rival those of any existing small, chemical inhibitors. We are continuing to work with these aptamers towards developing them into anti-excitotoxic drugs for treating patients with ALS, including those Gulf War veterans suffering from ALS.

Subject Categories:

  • Biochemistry
  • Anatomy and Physiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE