Accession Number:

ADA495861

Title:

In Vitro Toxicity of Silver Nanoparticles in Human Lung Epithelial Cells

Descriptive Note:

Master's Thesis

Corporate Author:

AIR FORCE INST OF TECH WRIGHT-PATTERSON AFB OH SCHOOL OF ENGINEERING AND MANAGEMENT

Personal Author(s):

Report Date:

2009-03-01

Pagination or Media Count:

99.0

Abstract:

Nanotechnology is quickly becoming incorporated into everyday products and uses. Silver nanoparticles, specifically, are being used in commercial products, to include aerosols. The purpose of this research was to determine whether silver nanoparticles are toxic to human lung epithelial cells. Different types coated vs. uncoated, concentrations 10, 50, 100, and 200 microgramsmL and sizes coated 5 and 80nm, uncoated 10 and 80nm of silver nanoparticles were used during this study. Toxicity measurements were completed through in vitro techniques. Another study was also completed on toxicity mechanisms by measuring the reactive oxygen species ROS generated. Results showed that silver nanoparticles induce mitochondrial toxicity through a size and concentration dependent manner. Increasing the concentration yielded increased toxicity and the smaller the size induced increased toxicity to the mitochondria. Results also showed that the uncoated nanoparticles were also more toxic to the cells than the coated nanoparticles. The small nanoparticles coated 5, uncoated 10nm induced more formation of the ROS than the larger nanoparticles 80nm.

Subject Categories:

  • Anatomy and Physiology
  • Medicine and Medical Research
  • Toxicology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE