Effects of Acute and Chronic Exercise on Disulfide-Linked Growth Hormone Variants
ARMY RESEARCH INST OF ENVIRONMENTAL MEDICINE NATICK MA MILITARY PERFORMANCE DIV
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Purpose To test the hypothesis that the appearance of disulfide-linked growth hormone GH aggregates during and after an acute resistance exercise test ARET in men could be influenced by chronic physical training. Fourteen men 28 or - 1 yr underwent two different 8-wk physical training programs designed to improve military performance. Before and after chronic training, subjects performed an ARET six sets of 10 repetition-maximum squat and had venous blood drawn pre-, mid-, and post-ARET 0, 15, and 30 min. post exercise. To determine whether GH molecules were disulfide-linked, serum samples were chemically reduced via glutathione GSH. Serum immunoreactive GH IRGH and immunofunctional GH IFGH concentrations were determined using two specific immunoassays, in nonreduced -GSH and reduced GSH states. Data were analyzed using repealed-measures ANOVA. Results No differences were observed in the GH responses of the two training programs therefore, training group data were combined for analysis.
- Anatomy and Physiology