In Vitro Correlate of Immunity in a Rabbit Model of Inhalational Anthrax
ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FORT DETRICK MD
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A serological correlate of vaccine-induced immunity was identified in the rabbit model of inhalational anthrax. Animals were inoculated intramuscularly at 0 and 4 weeks with varying doses of Anthrax Vaccine Adsorbed AVA ranging from a human dose to a 1256 dilution in phosphate-buffered saline PBS. At 6 and 10 weeks, both the quantitative anti-protective antigen PA IgG ELISA and the toxin-neutralizing antibody TNA assays were used to measure antibody levels to PA. Rabbits were aerosol-challenged at 10 weeks with a lethal dose 84-133 LD,, of Bacillus anthracis spores. All the rabbits that received the undiluted and 14 dilution of vaccine survived, whereas those receiving the higher dilutions of vaccine 116, 154 and 1256 had deaths in their groups. esultsh owed that antibody levels to PA at both 6 and 10 weeks were significant P 0.0001 predictors of survival.
- Anatomy and Physiology
- Medicine and Medical Research