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Enhancing Natural Attenuation Through Bioaugmentation with Aerobic Bacteria that Degrade Cis-1,2-Dichloroethene

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Monitored natural attenuation MNA and enhanced in situ bioremediation EISB remedies hold the promise of reducing the costs associated with cleanup of Department of Defense DoD sites impacted by chlorinated solvents. However, there are many DoD sites where tetrachloroethene PCE and trichloroethene TCE are only undergoing partial dechlorination to cis-1,2-dichloroethene cDCE, even when sufficient electron donor is present or added. As a result, there are a significant number of plumes at DoD and related sites where PCE and TCE have been dechlorinated to cDCE, but where the cDCE persists and migrates uncontrolled in groundwater rather than undergoing further dechlorination to ethene the desired end product. Through a project funded by the Strategic Environmental Research and Development Program SERDP project ER-1168 a novel aerobic bacterium JS666 was isolated and described that is able to use cDCE as a sole carbon and energy source under aerobic conditions, converting it to carbon dioxide and water without the addition of exotic co-factors Coleman et al., 2002a,b. Subsequently, the Environmental Security Technology Certification Program ESTCP funded additional work under project ER-0516 to optimize the growth of JS666, to further characterize its ability to degrade solvent mixtures, and to assess whether JS666 can transfer its ability to degrade cDCE to other in situ microorganisms. These studies were conducted in preparation for a field scale demonstration to evaluate the effectiveness of JS666 as an in situ bioaugmentation agent to degrade cDCE and other chlorinated solvents in groundwaters that are or will become aerobic. This laboratory study report presents the results of the Growth, Kinetic, and Optimization Assessment conducted under Task 1 of ESTCP project ER-0516.

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  • Microbiology
  • Solid Wastes and Pollution and Control
  • Water Pollution and Control

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