Accession Number:

ADA495317

Title:

Role of STAT5b in Breast Cancer Progression and Metastasis

Descriptive Note:

Annual summary, 1 Sep 2007-30 Aug 2008

Corporate Author:

VIRGINIA UNIV CHARLOTTESVILLE

Personal Author(s):

Report Date:

2008-09-01

Pagination or Media Count:

16.0

Abstract:

The signal transducer and activator of transcription STAT 5b is involved in tumorigenic signaling in a number of cancers including breast cancer. However, its importance in breast cancer migration has not been investigated. This research aims to determine the role of STAT5b in breast cancer migration. Knockdown of STAT5b inhibited migration of human breast cancer cells to serum by 60-80. This inhibition could be rescued by re-introduction of wild-type STAT5b as well as a transcriptionally inactive Y699F-STAT5b mutant. This suggests that the transcriptional activity of STAT5b is not required for its pro-migratory function. Knockdown of STAT1 also inhibited migration of MDA-MB-231 cells but knockdown of STAT3 or STAT5a had no effect. This data demonstrates that STAT5b is integral to breast cancer cell migration and that this effect may occur through a novel, non-transcriptional function. Further elucidating the mechanism by which STAT5b promotes migration of breast cancer cells may lead to more effective therapies for preventing and treating metastasis.

Subject Categories:

  • Genetic Engineering and Molecular Biology
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE