Maintaining Genome Stability: The Role of Helicases and Deaminases
Final rept. 1 Jul 2005-30 Jun 2008
UNIVERSITY OF SOUTHERN CALIFORNIA LOS ANGELES
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Both helicases and deaminases are enzymes that play an important role in maintaining genomic stability and immune responses. Errors in duplicating DNA can result in genomic instability, leading to various human diseases, such as cancer, immune system disorder, muscle dystrophy, and neurodegenerations. Thus, maintaining genomic integrity is vital to the normal growth of cells and to human health. Maintenance of genome integrity during S phase depends in part on the activity and regulation of the replication enzymes, including replicative helicase called the MCM complex to preserve the genetic information. In contrast, deliberate errors are introduced during maturation of the immune system by modifying enzymes called deaminases. The cells response to different forms of damage is fundamental to its ability to repair itself when challenged by environmental or chemical insults. The goal of this proposal is to investigate mechanisms of genome integrity in cells using a combined genetic, biochemical, and structural approaches. These studies are highly relevant to understanding the development of cancer, including leukemias and breast cancer.
- Genetic Engineering and Molecular Biology
- Medicine and Medical Research