Transcriptional Profiling of Francisella tularensis Infected Peripheral Blood Monomuclear Cells: A Predictive Tool for Tularemia
WALTER REED ARMY INST OF RESEARCH SILVER SPRING MD
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We studied early temporal gene expression in human peripheral blood mononuclear cells after in vitro infection with Francisella tularensisi live vaccine strain. Recently, investigators from Sweden studied ulceroglandular tularemia by gene expression profiling of blood from patients with active ongoing infection. Host responses to tularemia were described in their study of several potential genes as biomarkers for early tularemia. Most of the host genes related to tularemia were confirmed in our in vitro study, in addition to several biomarkers, indicating the value of gene expression profiling to identify potential biomarkers. Herein, we describe gene expression changes in the early human immune response and other cellular processes. We also report the identification of genes expressed in hitherto unknown pathways related to Francisella infection, notably those involved in zinc and lipid metabolism. Our work demonstrated the applicability of utilizing microarray gene expression as a predictor of in vivo immune responses to infectious agents.
- Medicine and Medical Research