Accession Number:

ADA488116

Title:

Evaluation of Quantitative Anti-F1 IgG and Anti-V IgG ELISAs for use as an in Vitro-Based Potency Assay of Plague Vaccine in Mice

Descriptive Note:

Journal article

Corporate Author:

ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FORT DETRICK MD BACTERIOLOGY DIV

Personal Author(s):

• Little, S. F.
• Webster, W. M.
• Wilhelm, H.
• Powell, B.
• Enama, J.
• Adamovicz, J. J.

Report Date:

2008-04-01

Pagination or Media Count:

10.0

Abstract:

Quantitative anti-F1 and anti-V IgG enzyme-linked immunosorbent assays ELISAs were developed to measure the serological response of female Swiss Webster mice after vaccination with the recombinant fusion protein, rF1-V, which is being developed as a plague vaccine. Several fundamental parameters of the ELISA were evaluated specificity, precision, accuracy, and stability. Experimental results suggested that a potency assay based upon the serological response of female Swiss Webster mice, as measured by quantitative anti-F1 IgG and anti-V IgG ELISAs, might be used to evaluate the rF1-V fusion protein vaccine.

Descriptors:

• *YERSINIA PESTIS
• *PLAGUE VACCINE
• *IN VITRO ANALYSIS
• IMMUNOASSAY
• SEROLOGY
• POTENCY
• FEMALES
• VACCINES
• LABORATORY ANIMALS
• MICE
• ENZYMES
• REPRINTS
• IMMUNIZATION

Subject Categories:

• Anatomy and Physiology
• Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE