Accession Number:
ADA488116
Title:
Evaluation of Quantitative Anti-F1 IgG and Anti-V IgG ELISAs for use as an in Vitro-Based Potency Assay of Plague Vaccine in Mice
Descriptive Note:
Journal article
Corporate Author:
ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FORT DETRICK MD BACTERIOLOGY DIV
Personal Author(s):
Report Date:
2008-04-01
Pagination or Media Count:
10.0
Abstract:
Quantitative anti-F1 and anti-V IgG enzyme-linked immunosorbent assays ELISAs were developed to measure the serological response of female Swiss Webster mice after vaccination with the recombinant fusion protein, rF1-V, which is being developed as a plague vaccine. Several fundamental parameters of the ELISA were evaluated specificity, precision, accuracy, and stability. Experimental results suggested that a potency assay based upon the serological response of female Swiss Webster mice, as measured by quantitative anti-F1 IgG and anti-V IgG ELISAs, might be used to evaluate the rF1-V fusion protein vaccine.
Descriptors:
Subject Categories:
- Anatomy and Physiology
- Medicine and Medical Research