Exploiting for Breast Cancer Control a Proposed Unified Mechanism for Reduction of Human Breast Cancer Risk by the Hormones of Pregnancy
Final rept. 5 Apr 2004-4 Apr 2008
ALBANY MEDICAL COLL NY
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Results in the third grant year further support the Unified Mechanism Hypothesis in that 1 Giving pregnancy associated hormones or hCG to virgin female rats either before or after MNU treatment elicits persistent serum AFP levels, thereby explaining why breast cancer appearance is inhibited when employing either treatment sequence 2 Giving hCG to estrogenized SCID mice bearing human breast cancer xenografts inhibits the cancer growth, apparently by action of murine AFP that the treatment has elicited from the mouse liver as an inform with low avidity for our available anti mAFP antibody. We are able to detect mAFP in mouse serum by western blot when it is present in the very high concentration that is elicited by injection of the animals with high E3 doses. 3 In an all-human in vitro system, hCG elicits hAFP from cultured HepG2 human liver cancer cells, addition of the hAFP-containing supernate to cultures of MCF7 human breast cancer cells blocks their growth, and that adding anti hAFP antibody to that system prevents the inhibition. hAFP is thus confirmed as the proximal inhibitor.
- Medicine and Medical Research