Genes Involved in Oxidation and Prostate Cancer Progression
Final addendum rept. 14 Dec 2006-14 Dec 2007
JOHNS HOPKINS UNIV BALTIMORE MD BLOOMBERG SCHOOL OF PUBLIC HEALTH
Pagination or Media Count:
We are evaluating whether polymorphisms in genes involved in the genesis of oxidative species, detoxification of oxidative species, or repair of oxidative DNA damage influence risk of prostate cancer progression in men with clinically organ-confined prostate cancer. We identified 524 men with who underwent radical prostatectomy in 1993-2004 and who subsequently experienced biochemical recurrence, development metastases, or died from their prostate cancer. Using incidence-density sampling, we selected 524 men matched on age, race, and pathological stage and grade who had not progressed by the date of the matched cases progression. Noncancer tissue either unaffected paraffin-embedded lymph nodes or frozen seminal vesicles was retrieved from the Hopkins pathology archive from which germline DNA was extracted. For 20 men either tissue could be found or DNA extraction was not successful. We attempted several platforms for genotyping, including a SNP chip that included 1500 SNPs in relevant genes. We selected the Mass Array system Sequenom and identified 100 SNPs in relevant genes. We completed genotyping the 524 pairs for 12 of the 100 SNPs. For 33 of the men, genotyping was not successful. A total of 450 of the 524 pairs had genotype data for both members of the pair for at least on SNP. We used conditional logistic regression to estimate the matched ORs of progression for the 12 SNPs no associations were observed all p-trend across number of alleles 0.15. We are awaiting data for the remainder of the 100 SNPs.
- Genetic Engineering and Molecular Biology
- Medicine and Medical Research