Accession Number:

ADA482921

Title:

A Novel Approach to the Development of Highly Specific Inhibitors of EFG, a Critical Transcription Factor in Prostate Cancer

Descriptive Note:

Annual rept. 15 Feb 2007-14 Feb 2008

Corporate Author:

VIRGINIA UNIV CHARLOTTESVILLE

Report Date:

2008-03-01

Pagination or Media Count:

6.0

Abstract:

We have created a series of six constructs to express varying length fragments of ERG. Expression and purification protocols have been established for all six fragments. Using fluorescein-labeled oligonucleotides, we have developed a fluorescence polarization based assay for measuring the DNA binding of the ERG fragments to a functional DNA element. Using this assay, we have shown that longer fragments of ERG show decreased DNA binding, i.e. are auto-inhibited. Based on this data, we have focused efforts on producing one fragment in the labeled form necessary for NMR structural studies.

Subject Categories:

  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE