A Myc-Driven in Vivo Model of Human Prostate Cancer
Final rept. 15 Sep 2004-14 Sep 2007
VANDERBILT UNIV MEDICAL CENTER NASHVILLE TN
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The long-term goal of the work proposed here is to generate, characterize and interrogate human epithelial cell-based in vivo models of prostatic carcinogenesis. These models will allow an examination of processes involved in carcinogenesis, tumor growth and metastasis. Since the tumors are themselves of human origin they represent an in vivo test bed to examine both tumor biology and the application of therapeutic agents. In the third year of funding we have completed a thorough study of metastatic spread of human prostatic epithelium from the orthotopic site using a model developed in year 2. We have generated and characterized two new normal prostatic epithelial cell lines which show promise for widespread applicability in prostate cancer research. We have continued to explore the use of lower dose Myc expressing constructs and have investigated the combination of lower levels of Myc with additional other genes commonly changed in prostate cancer to make more clinically relevant models. Specifically we have followed up on the suppression of PTEN described in the second report and have also combined suppression of PPAR. with expression of cMyc to produce enhancement of a mild myc-induced phenotype.
- Anatomy and Physiology
- Medicine and Medical Research