Activation and Protection of Dendritic Cells in the Prostate Cancer Environment
Annual rept. 16 Jan 2007-14 Jan 2008
UNIVERSITY OF MEDICINE AND DENTISTRY OF NEW JERSEY NEWARK
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Third annual report for this award. Experiments were conducted as was scheduled in the Statement of Work. So far studies have demonstrated for the first time the presence of endothelin receptors on murine DC, and the fact of endothelin-1 production by murine DC upon stimulation with TNF- and lipopolysaccharide LPS. The modification of the endothelin axis on DC changed its resistance against prostate cancer induced apoptosis the blockade of ETA receptors resulted in the increased apoptotic rate, while the blockade of the ETB receptors lead to the increased survival of DC in the prostate cancer environment. Based on these data, in vivo experiments were carried out, in which mice with prostate cancer RM1 cells were treated with intratumoral injection of modified DC stimulated DC, with ETB receptor blocked. This treatment resulted in reduction of prostate cancer growth in mice in the experimental group, in comparison to untreated control mice. Studies are under way to elicit the mechanisms of endothelin axis action on DC, as well as the underlying mechanisms of interaction between dendritic cells and prostate cancer cells.
- Anatomy and Physiology
- Medicine and Medical Research