Accession Number:

ADA482634

Title:

Mechanism of Xanthine Oxidase Catalyzed Biotransformation of HMX Under Anaerobic Conditions

Descriptive Note:

Corporate Author:

NATIONAL RESEARCH COUNCIL OF CANADA MONTREAL (QUEBEC) BIOTECHNOLOGY RESEARCH INST

Report Date:

2003-01-01

Pagination or Media Count:

8.0

Abstract:

Enzyme catalyzed biotransformation of the energetic chemical octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine HMX is not known. The present study describes a xanthine oxidase XO catalyzed biotransformation of HMX to provide insight into the biodegradation pathway of this energetic chemical. The rates of biotransformation under aerobic and anaerobic conditions were 1.6 0.2 and 10.5 0.9 nmol h 1 mg protein 1, respectively, indicating that anaerobic conditions favored the reaction. The biotransformation rate was about 6-fold higher using NADH as an electron-donor compared to xanthine. During the course of reaction, the products obtained were nitrite NO , methylenedinitramine MDNA, 4-nitro-2,4-diazabutanal NDAB, formaldehyde HCHO, nitrous oxide N2O, formic acid HCOOH, and ammonium NH4 . The product distribution gave carbon and nitrogen mass-balances of 91 and 88, respectively. A comparative study with native-, deflavo-, and desulfo-XO and the site-specific inhibition studies showed that HMX biotransformation occurred at the FAD-site of XO. Nitrite stoichiometry revealed that an initial single N-denitration step was su cient for the spontaneous decomposition of HMX.

Subject Categories:

  • Biochemistry
  • Inorganic Chemistry
  • Ammunition and Explosives

Distribution Statement:

APPROVED FOR PUBLIC RELEASE