Development of a Novel Vaccine Vector for Multiple CDC Category A Pathogens
Final rept. 1 Apr 2007-31 Mar 2008
OREGON HEALTH AND SCIENCES UNIV PORTLAND
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Specific Aim 1 was to generate a panel of RhCMVMPV vectors expressing MPV antigens A29L, A35R, M1R and B6R in either the wild type RhCMV vector, or in a vector lacking MHC immunomodulatory genes. To date, two vectors have been constructed and characterized, and we have subsequently selected one WT RhCMV vector WTRhCMVA35R for immunogical characterization in rhesus macaques. Specific Aims 2 and 3 were to establish the pathobiology of WT MPV infection in RMs, and to monitor the immunological consequences of WT MPV infection. To date, eight RMs have been experimentally inoculated intrabronchially with MPV Zaire strain MPVZ to define a lethal dose by this route of infection and to characterize the virushost interactions. Together, completion of these three specific aims will form the foundation for future studies designed to determine the efficacy of the RhCMVMPV vectors at inducing a protective immune response to MPV challenge in RMs, and to identify viral determinants of pathogenesis.
- Medicine and Medical Research