New Materials Based on Spider Silk
Final rept. 15 Sep 2006-14 Sep 2007
TECHNICAL UNIV MUNICH (GERMANY)
Pagination or Media Count:
In this project, we investigated the dragline silk proteins ADF-3 and ADF-4 of the spider Araneus diadematus. Adf-3 and Adf-4 cDNA can each be expressed directly in insect cells using the Baculovirus-expression-System. Recombinant ADF-4 is insolubly produced in the cytoplasm of insect cells and soluble produced upon secretion into the media. Further, parameters influencing stability and solubility of recombinantly produced ADF analogues have been investigated. We detected that intermolecular disulfide bridges formed between monomers of the ADF analogues stabilized formed dimer. Additionally, it was found that potassium phosphate triggers assembly of ADF analogues into spheres and fibrils.