Accession Number:

ADA482569

Title:

New Materials Based on Spider Silk

Descriptive Note:

Final rept. 15 Sep 2006-14 Sep 2007

Corporate Author:

TECHNICAL UNIV MUNICH (GERMANY)

Personal Author(s):

Report Date:

2007-11-06

Pagination or Media Count:

14.0

Abstract:

In this project, we investigated the dragline silk proteins ADF-3 and ADF-4 of the spider Araneus diadematus. Adf-3 and Adf-4 cDNA can each be expressed directly in insect cells using the Baculovirus-expression-System. Recombinant ADF-4 is insolubly produced in the cytoplasm of insect cells and soluble produced upon secretion into the media. Further, parameters influencing stability and solubility of recombinantly produced ADF analogues have been investigated. We detected that intermolecular disulfide bridges formed between monomers of the ADF analogues stabilized formed dimer. Additionally, it was found that potassium phosphate triggers assembly of ADF analogues into spheres and fibrils.

Subject Categories:

  • Biology
  • Textiles
  • Thermodynamics

Distribution Statement:

APPROVED FOR PUBLIC RELEASE