Investigating the Functional Role of Prostate-Specific Membrane Antigen and its Enzymatic Activity in Prostate Cancer Metastasis
Annual rept. 30 Jan 2007-28 Jan 2008
GENERAL HOSPITAL CORP BOSTON MA DBA MASSACHUSETTS GENERAL HOSPITAL BOSTON
Pagination or Media Count:
It is fundamentally important to understand the underlying mechanisms regulating prostate caner PCa metastasis. Despite the increased PSMA expression found in more advanced stage of PC a little is known about the functional role of PSMA in PCa progression. Work accomplished for the period of the report has 1 generated the fluorescently labeled anti-PSMA antibodies for monitoring PSMA expressions in live PCa cells 2 established the cell model systems with reduced PSMA expression for studying PSMA functions 3 identified fibronetin as a specific extracellular matrix for enhanced LNCaP attachment and 4 performed 2-D wound healing assays to examine the role of PSMA in PCa cell migration. Results from these studies demonstrated and further supported the idea that PSMA is involved in PCa cell adhesion and migration therefore will enhance our understanding of molecular regulatory mechanisms of PCa. Knowledge about the action of PSMA in cell adhesion and migration during PCa metastasis will have a direct impact on the improvement for design better-targeted approaches for treating patients suffering from metastatic prostate cancer.
- Medicine and Medical Research