Investigation of Viability of Pantoea agglomerans (Formerly Erwinia herbicola) After Aerosolization From Media Containing Enriching and Coating Chemicals
Final rept. Sep 2005-Sep 2006
JOHNS HOPKINS UNIV BALTIMORE MD BLOOMBERG SCHOOL OF PUBLIC HEALTH
Pagination or Media Count:
Percent viability of the sensitive bacteria Pantoea agglomerans ATCC33243, formerly Erwinia herbicola or Eh, is an important issue in aerosol testing of vegetative cells, which might be found in the environment. The viability of this bacterial strain has usually been poor during aerosol testing. However, we have found a number of examples in the literature where outdoor testing with various strains of this bacterium apparently was improved with certain additives before or after bacteria growth. We have been testing aerosol survival of this strain using various components found in published recipes to improve its effectiveness as a test organism. We report on tests performed with bacteria grown and aerosolized from Tryptic soy broth TSB, TSB with sugar additives sucrose and trehalose, and aerosolized from these growth media after centrifuge-washing with water. We also report experiments of aerosolized bacteria treated with glycerin and with glycerin plus Na-alginate GNA under various conditions. We found improved survival for the bacteria dried on a surface, but were not able to obtain consistent results in the time allotted to the project when the bacteria were aerosolized.
- Chemical, Biological and Radiological Warfare