Accession Number:
ADA478963
Title:
Rapid Active Assay for the Detection of Antibodies to West Nile Virus in Chickens
Descriptive Note:
Journal article
Corporate Author:
GEORGE MASON UNIV MANASSAS VA NATIONAL CENTER FOR BIODEFENSE AND INFECTIOUS DISEASE
Personal Author(s):
Report Date:
2008-01-01
Pagination or Media Count:
8.0
Abstract:
To reduce the assay time for detecting virus-specific antibodies in serum, we developed microarray-based active immunoassay techniques for detecting West Nile virus WNV-specific IgM molecules in chicken blood. The assay uses electrophoretic concentration of IgM molecules onto WNV antigens arrayed on a dialysis membrane followed by detection of bound IgM molecules with functionalized magnetic beads as active labels. This assay takes only 15 minutes and has the same sensitivity as a commercially available human WNV IgM antibody-capture enzyme-linked immunosorbent assay commonly called a MAC-ELISA modified for use with chicken sera.
Descriptors:
Subject Categories:
- Biochemistry
- Medicine and Medical Research
- Microbiology