Accession Number:

ADA478963

Title:

Rapid Active Assay for the Detection of Antibodies to West Nile Virus in Chickens

Descriptive Note:

Journal article

Corporate Author:

GEORGE MASON UNIV MANASSAS VA NATIONAL CENTER FOR BIODEFENSE AND INFECTIOUS DISEASE

Report Date:

2008-01-01

Pagination or Media Count:

8.0

Abstract:

To reduce the assay time for detecting virus-specific antibodies in serum, we developed microarray-based active immunoassay techniques for detecting West Nile virus WNV-specific IgM molecules in chicken blood. The assay uses electrophoretic concentration of IgM molecules onto WNV antigens arrayed on a dialysis membrane followed by detection of bound IgM molecules with functionalized magnetic beads as active labels. This assay takes only 15 minutes and has the same sensitivity as a commercially available human WNV IgM antibody-capture enzyme-linked immunosorbent assay commonly called a MAC-ELISA modified for use with chicken sera.

Subject Categories:

  • Biochemistry
  • Medicine and Medical Research
  • Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE