Rapid Active Assay for the Detection of Antibodies to West Nile Virus in Chickens
GEORGE MASON UNIV MANASSAS VA NATIONAL CENTER FOR BIODEFENSE AND INFECTIOUS DISEASE
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To reduce the assay time for detecting virus-specific antibodies in serum, we developed microarray-based active immunoassay techniques for detecting West Nile virus WNV-specific IgM molecules in chicken blood. The assay uses electrophoretic concentration of IgM molecules onto WNV antigens arrayed on a dialysis membrane followed by detection of bound IgM molecules with functionalized magnetic beads as active labels. This assay takes only 15 minutes and has the same sensitivity as a commercially available human WNV IgM antibody-capture enzyme-linked immunosorbent assay commonly called a MAC-ELISA modified for use with chicken sera.
- Medicine and Medical Research