Prostate Derived Ets Factor, An Immunogenic Breast Cancer Antigen
Final rept. 1 Sep 2005-31 Aug 2007
ROSWELL PARK CANCER INST BUFFALO NY
Pagination or Media Count:
The goal of the proposed research was to test the following concept Due to the prostate restricted expression of Pse in normal tissues of mice Pse is likely to be immunogenic in female mice. T cell responses of male and female mice to Pse and a control antigen Her2lneu were compared. Two different assays were used for this purpose the ELlspot assay and the Cytotoxicity assay. We found that in the FVB and C57BL16 strains of mice immunization with Pse induced specific T cell responses in the female mice but showed no or weak responses in the male mice. In contrnst the difference in T cell responses of male and female mice to immunization with Her2lneu antigen was relatively small. These results are novel and highly significant and they support our concept that uncharacteristically high difference in responsiveness to Pse of female mice in comparison to male mice is due to the lack of significant expression of Pse as a self-antigen in female mice. Further these results suggest that PDEF the human homologue of Pse is likely to be significantly immunogenic in female breast cancer patients. The observed poor immunogenicity of Pse in the male mice is probably due to some mechanism of tolerance against this protein due to its strong expression in the prostate gland.
- Anatomy and Physiology
- Medicine and Medical Research
- Stress Physiology