Accession Number:

ADA477669

Title:

Incubation and Growth of Life Sciences, Medical and Biotechnology Businesses in Proteomics, Genomics, Medicine, and Dentistry

Descriptive Note:

Final rept. 28 Sep 2005-30 Mar 2007

Corporate Author:

ADVANCED RESEARCH AND TECHNOLOGY INST INDIANAPOLIS IN

Report Date:

2007-04-01

Pagination or Media Count:

46.0

Abstract:

Trace level detection of chemical warfare agent simulants and biological toxins by desorption electrospray ionization DESI has been demonstrated. The detection of several chemical agent simulants as well as peptide and fungal toxins was shown at picogram levels from a variety of surfaces and in the presence of potential matrix interferences. In addition the detection of intact bacterial cells was also demonstrated. Smears of cells taken from cultures were analyzed yielding characteristic mass spectra for the different species studied. Ions arising from samples of Pseudomonas aeruginosa have been successfully identified as quinoline intercellular signaling molecules. Ions from other species have not yet been identified. Finally a prototype DESI wand was developed for the sampling of object not accessible by the standard mass spectrometer interface. The device extended approximately 20 cm from the mass spectrometer and was equipped with an array of both DESI spray heads and ion collection tubes enabling higher surface area scanning than is possible with a single spray headion collection tube combination

Subject Categories:

  • Medicine and Medical Research
  • Chemical, Biological and Radiological Warfare

Distribution Statement:

APPROVED FOR PUBLIC RELEASE