NF-1 Dependent Gene Regulation in Drosophila Melanogaster
Final rept. 1 Apr 2002-31 Mar 2004
COLD SPRING HARBOR LAB NY
Pagination or Media Count:
Neurofibromatosis type 1 NF1 is characterized by benign but disfiguring skin tumors, pigmentation defects and learning disabilities, as well as increased risk of brain tumors. The NF1 tumor suppressor protein neurofibromin inhibits Ras, a protein that is overactive in a wide variety of human cancers. NF1 also controls levels of cyclic AMP, an important intracellular messenger involved in cell growth and learning. We have used an Affymetrix whole genome chip, containing all 13,500 genes of the fruit fly Drosophila, to identify 93 genes with altered expression patterns in flies that have no NF1 protein compared to normal wild type flies. Importantly, expression of half these genes is restored to normal levels when human NF1 is expressed in flies with no NF1. At least a quarter of these genes are involved in nervous system function or development.
- Medicine and Medical Research