Functional Characterization of a Novel Pro-Apoptotic Transcription Regulatory Protein in Ovarian Cancer
Final rept. 15 Dec 2003-14 Nov 2006
MAYO CLINIC ROCHESTER MN
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In an effort to identify genetic changes involved in ovarian cancer OvCa development, we performed differential display-PCR, cDNA microarray and suppression subtraction hybridization analyses SHH to identify early genetic alterations associated with OvCa. These studies resulted in identification of several genes differentially expressed in OvCa, including a novel gene encoding a transcription elongation-like protein with the ability to induce apoptosis and suppress cancer cell growth. We named the protein ProApoptotic Protein on chromosome X PAPX. Pro-apoptotic protein on X PAPX is a novel nuclear protein with sequence homology to transcription elongation factor like 1 TCEAL1 1. PAPX expression is down-regulated in majority of ovarian cancer cell lines and primary tumors 2. Re-expression of PAPX induces cell death and attenuates cell growth. We therefore proposed to study the functional role of PAPX as a candidate tumor suppressor in ovarian cancer. We proposed to 1 determine effect of PAPX on tumor and cell growth in vivo and in vitro 2 analyze genes regulated by PAPX by transcriptional profiling using microarray chips and 3 identify proteins that interact with PAPX and elucidate the function of PAPX related to tumor suppression.
- Genetic Engineering and Molecular Biology
- Medicine and Medical Research