Accession Number:

ADA470738

Title:

Damage Thresholds for Cultures RPE Cells Exposed to Lasers at 532 nm and 458 nm

Descriptive Note:

Journal Article

Corporate Author:

NORTHROP GRUMMAN SAN ANTONIO TX WARFIGHTER CONCEPTS AND APPLICATIONS DEPT

Report Date:

2007-06-01

Pagination or Media Count:

9.0

Abstract:

The determination of safe exposure levels for lasers has come from damage assessment experiments in live animals, which typically involve correlating visually identifiable damage with laser dosimetry. Studying basic mechanisms of laser damage in animal retinal systems often requires tissue sampling animal sacrifice, making justification and animal availability problematic. We determined laser damage thresholds in cultured monolayers of a human retinal pigment epithelial RPE cell line. By varying exposure duration and laser wavelength, we identified conditions leading to damage by presumed photochemical or thermal mechanisms. A comparison with literature values for ocular damage thresholds validates the in vitro model. The in vitro system described will facilitate molecular and cellular approaches for understanding laser-tissue interaction.

Subject Categories:

  • Anatomy and Physiology
  • Weapons Effects (Biological)
  • Lasers and Masers
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE