Hyaluronan Biosynthesis in Prostate Carcinoma
Final rept. 1 Jan 2002-31 Dec 2006
MINNESOTA UNIV MINNEAPOLIS
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Despite advances in the diagnosis and treatment of prostate cancer in the last several years, metastasis represents the major cause of frustration and failure in the successful treatment of prostate cancer patients. Hyaluronan HA is polymeric anionic carbohydrate that is elevated within primary prostate tumors, most notably within the tumor-associated stroma. We have demonstrated that increased HA synthesis by human prostate carcinoma cells correlates with metastatic potential. This increased synthesis results from the elevated expression of specific hyaluronan synthases HAS in the tumor cells. Metastatic prostate carcinoma cells exhibiting high levels of HAS assemble and retain a pericellular HA matrix on their cell surfaces. We have used vectors to stably express constructs encoding antisense for HAS enzymes to study the importance of elevated hyaluronan synthesis in prostate carcinoma adhesion, growth and tumor formation. The studies outlined in this annual report document our observations that support an important role for hyaluronan in prostate tumor progression.
- Anatomy and Physiology