The Role of ABC Proteins in Drug-Resistant Breast Cancer Cells
Annual summary rept. 15 Mar 2006-14 Mar 2007
GEORGETOWN UNIV WASHINGTON DC DEPT OF BIOCHEMISTRY
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This predoctoral training grant supported by the Department of Defense Breast Cancer Research Program BCRP of the Office of the Congressionally Directed Medical Research Programs CDMRP aims to study the possible role of ABC transporters in altered apoptotic signal transduction in drug sensitive and resistant cells by a combination of molecular biological biochemical and biophysical methods. The first year of the funding period was spent learning basic molecular biology techniques while working with a model system. The plasmodial homologue of human Pgp PfMDR1 was heterologously expressed in yeast and its function in response to drugs measured in terms of ATPase activity. The data from this project have already led to a quality journal publication with more forthcoming and the knowledge and skills I have gained will undoubtedly be extremely valuable in future endeavors with human Pgp. Preparations in terms of mammalian cell culture and microscopy methodology have been made for the start of actual Pgp research and the timetable as outlined in the original grant proposal is still intact.
- Medicine and Medical Research