Accession Number:

ADA470177

Title:

Development of Effective Aerobic Cometabolic Systems for the In Situ Transformation of Problematic Chlorinated Solvent Mixtures

Descriptive Note:

Final rept.

Corporate Author:

OREGON STATE UNIV CORVALLIS DEPT OF CIVIL CONSTRUCTION AND ENVIRONMENTAL ENGINEERING

Report Date:

2005-02-01

Pagination or Media Count:

425.0

Abstract:

Many sites in the DoD and DOE complex are contaminated with chlorinated solvent mixtures. Passive in-situ treatment via aerobic cometabolism is one means of potentially restoring the contaminated aquifers. The goal of the project was to develop a cometabolic culture that has potential for bioaugmentation and to evaluate its performance under laboratory conditions and under in-situ conditions in field demonstrations. The specific objectives of this study were to 1 develop a butane utilizing culture for bioaugmentation in laboratory and field experiments 2 characterize the bioaugmentation culture using molecular methods, including a clone library, sequencing, and PCR based methods 3 develop kinetic information for substrate utilization and the transformation of the CAH mixtures 4 develop molecular based methods for tracking the cultures that were bioaugmented and biostimulated in laboratory and field studies 5 conduct laboratory microcosm and continuous flow column studies to evaluate the performance of the bioaugmented culture under geochemical conditions mimicking those present at the field site 6 conduct field demonstrations to evaluate the bioaugmentation approach and to determine the effectiveness in treating problematic mixtures of 1,1,1-TCA, 1,1-DCE, and 1,1-DCA using butane as cometabolic substrate, and compare the results to those achieved by indigenous butane-utilizers 7 track microbial community changes and quantify members of the bioaugmented culture in situ using molecular based methods 8 simulate the results of laboratory and field studies using a transport code for these cometabolic transformations.

Subject Categories:

  • Biochemistry
  • Physical Chemistry
  • Water Pollution and Control
  • Ecology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE