Development of Effective Aerobic Cometabolic Systems for the In Situ Transformation of Problematic Chlorinated Solvent Mixtures
OREGON STATE UNIV CORVALLIS DEPT OF CIVIL CONSTRUCTION AND ENVIRONMENTAL ENGINEERING
Pagination or Media Count:
Many sites in the DoD and DOE complex are contaminated with chlorinated solvent mixtures. Passive in-situ treatment via aerobic cometabolism is one means of potentially restoring the contaminated aquifers. The goal of the project was to develop a cometabolic culture that has potential for bioaugmentation and to evaluate its performance under laboratory conditions and under in-situ conditions in field demonstrations. The specific objectives of this study were to 1 develop a butane utilizing culture for bioaugmentation in laboratory and field experiments 2 characterize the bioaugmentation culture using molecular methods, including a clone library, sequencing, and PCR based methods 3 develop kinetic information for substrate utilization and the transformation of the CAH mixtures 4 develop molecular based methods for tracking the cultures that were bioaugmented and biostimulated in laboratory and field studies 5 conduct laboratory microcosm and continuous flow column studies to evaluate the performance of the bioaugmented culture under geochemical conditions mimicking those present at the field site 6 conduct field demonstrations to evaluate the bioaugmentation approach and to determine the effectiveness in treating problematic mixtures of 1,1,1-TCA, 1,1-DCE, and 1,1-DCA using butane as cometabolic substrate, and compare the results to those achieved by indigenous butane-utilizers 7 track microbial community changes and quantify members of the bioaugmented culture in situ using molecular based methods 8 simulate the results of laboratory and field studies using a transport code for these cometabolic transformations.
- Physical Chemistry
- Water Pollution and Control