Identification of Mechanisms of Breast Cancer Metastasis Using Tissue Specific Virus Delivery
Annual summary rept. 15 Feb 2006-14 Feb 2007
SLOAN-KETTERING INST FOR CANCER RESEARCH NEW YORK
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To investigate breast cancer metastasis we propose to use a tissue-specific viral delivery system that will allow the somatic delivery of secondary lesions in the background of a tumor-inducing primary oncogene. In the first year of support we established protocols for efficient in vitro cultivation and infection of mammary gland-derived normal hyperplastic and tumorigenic cells. For the second year we started to analyze the behavior of mammary tumor cells when infected with candidate genes that are likely to promote invasion. We analyzed infected cells in mammary fat pad re-implantation assays and established a three dimensional basement membrane-based cell culture system for primary mouse mammary cells that will allow us to examine differences after infection of normal hyperplasic tumorigenic and invasive mammary cells in more detail.
- Anatomy and Physiology
- Medicine and Medical Research