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Metabolic Mapping of Breast Cancer with Multiphoton Spectral and Lifetime Imaging
Annual rept. 1 Mar 2006-28 Feb 2007
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Currently we designed and implemented a combined spectral and lifetime imaging system which combines the benefits of multiphoton microscopy, spectral discrimination, lifetime analysis and temporal information. This system has efficiencies of 50 and capability to collect broad spectrums from 380nm-700nm. We determined the fluorescence source under our imaging condition is from NADH by this combined spectral and lifetime imaging system. Furthermore, we validated the accuracy of our lifetime analysis method. Our preliminary results of metabolic mapping in different human breast cell lines via fluorescence lifetime measurement of Co-Enzyme NADH showed very interesting results in differences of the lifetime and contribution of bound NADH between human normal breast cell line MCF10a and human breast cancer cell lines T47D and MBAMD231. A 2-Deoxy-D-glucose 2DG perturbation study is underway to link these finds with glycolysis.
APPROVED FOR PUBLIC RELEASE