Functional Characterization of Two Novel Human Prostate Cancer Metastasis Related Genes
Final rept. 16 Jan 2004-15 Jan 2007
TULANE UNIV NEW ORLEANS LA
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We propose to identify the functional characterization of two novel cancer-specific, metastasis-related genes whose constitutive expression may be pivotal for prostate cancer progression. Due to hurricane Katrina, we were able to gain access to research laboratories in end of April, 2006. Due to prolonged power outages in our building approximately 7 months, we have lost all of DNA constructs, cell lines, frozen tissue sections, cell lines, research supplies. In addition, all of our laboratory personnel were relocated to other cities following hurricane Katrina. Accordingly, we spent considerable amount of time June, 2006 through January, 2007 cleaning up labs, replacing laboratory supplies and equipments, and hiring lab personnel. Over the past three months we were able to re-generate Seq1 and Seq 2 DNA construct using approaches shown above. We have characterized the full-length cDNAs of the Seq1 and Seq2 genes using at least three 5 and 3 rapid amplification of cDNA ends RACE commercial kits Invitrogen Carlsbad, CA, BD Bioscience Clontech Inc, and Seegene, Rockville, MD. We have also subcloned cDNAs in expression plasmids and were sequence verified for orientation and recombinant protein expression, in vitro translation, and antibody production. However, our research plans of development of antibodies and validation of expression in tissue sections were disrupted by hurricane Katrina and destruction of all of our laboratory materials and supplies and experimental animals. To accomplish this, we have requested a one year extension to complete the remaining tasks.
- Anatomy and Physiology
- Medicine and Medical Research