Midkine Promoter-Driven Adenovirus as Potential Therapy for NF1
Final rept. 21 Nov 2005-20 Nov 2006
CALIFORNIA UNIV SAN FRANCISCO
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We proposed to test the possibility of using midkine promoter driven adenovirus AdMk to selectively kill neurofibroma cells. It is suggested from the literature that midkine is expressed at higher levels in cells and sera of NF1 patients. We initially obtained a version of the AdMk and tested its cytopathic effect on a neurofibroma cell line ST88. However a more recent publication indicated that this virus would be too toxic for normal cells as well. We then obtained from our new collaborator several different modified adenovirus and tested their cytopathic effects. Promoter activities of the midkine and Cox2 are also assayed in several cell lines.
- Medicine and Medical Research