Imaging Primary Prostate Cancer and Bone Metastasis
Annual rept. 1 Apr 2004-31 Mar 2006
STANFORD UNIV CA
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The overall objective of the proposed research is to develop positron emitter labeled bombesin BBN analogs with high affinity for the GRP receptor GRPR for microPET imaging of both androgen dependent and androgen independent prostate cancer xenografted mice. Specific Aims 1 Design synthesize and characterize positron emitting bombesin analogs labeled with copper-64 or fluorine-18 2 Conduct in vitro studies of copper-64 and fluorine-18 labeled bombesin analogs to evaluate the effect of modification and radiolabeling on the receptor binding affinity and specificity 3 Evaluate in vivo efficacy of these novel radiopharmaceuticals in the murine PC-3 and CWR22 human prostate cancer xenograft models. Major Findings In year 1 we coupled Lys3-BBN with DOTA and labeled the DOTA-Lys3-BBN conjugate with 64Cu for imaging both subcutaneous PC-3 GRPR and CWR22 GRPR- tumors. In year 2 we further tested a series of BBN analogs and fully characterized 64Cu-DOTA-Aca-BBN7-14. Studies on metabolic stability for both tracers on organ homogenates showed that 64Cu-DOTA-Lys3BBN is relatively stable. This study demonstrated that both tracers are suitable for targeted PET imaging to detect the expression of GRPR in prostate cancer while 64Cu-DOTA-Lys3BBN may have a better potential for clinical translation.
- Medicine and Medical Research