High-Throughput Analysis of Dynamic Gene Expression Associated with Sleep Deprivation and Recovery Sleep in the Mouse Brain
Annual rept. 1 Dec 2005-30 Nov 2006
ALLEN INST FOR BRAIN SCIENCE SEATTLE WA
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Sleep deprivation in mice causes changes in gene expression in discrete brain regions associated with sleepwake control as well as higher cognitive functions. Laser capture microdissection was used to isolate a set of relevant brain regions, and these samples were amplified to allow genome-wide genetic profiling on DNA microarrays to search for altered gene expression correlated with sleeping, waking, sleep deprivation and recovery sleep following sleep deprivation. Candidate genes from this microarray approach have been comprehensively analyzed using a highthroughput in situ hybridization platform. The resulting data indicate that there is dynamic gene expression correlated with circadian time of day, sleeping vs. waking states, and sleep deprivation specifically. This dynamic gene expression is also highly brain region and cell type-specific within a given region. These genes help to provide an anatomical and genetic framework for understanding the consequences of sleep deprivation and potential new means for pharmacological intervention. A web-based viewer interface has been developed to provide access to this data for the entire sleep community.
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