The Effects of Repeated Sub-Toxic Sarin Exposure on Behavior, EEG and Blood and Brain AChE Activity
Technical rept. Oct 2000-Sep 2003
ARMY MEDICAL RESEARCH INST OF CHEMICAL DEFENSE ABERDEEN PROVING GROUND MD
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Guinea pigs were exposed 5 daysweektwo weeks to saline or one of two sarin doses 0.2 or 0.4 x LD50. Animals were assessed for signs of toxicity, changes in body weight and temperature, EEG activity, behavioral and biochemical parameters during exposure E1-10 and up to 100 days post-exposure P100. Red blood cell AChE was depressed 60 and 80 respectively in the 0.2 and 0.4 x LD50 groups following E10, returning to baseline by P100. Brain AChE was significantly reduced in the 0.4 x LD50 animals only following E10, returning to baseline by P100. The 0.4 x LD50 animals exhibited a significant increase in beta2 EEG activity across exposure days, but returned to control levels by P3. No differences were noted among groups on toxicity signs, weight or temperature. A significant increase in flinch threshold in both sarin groups was found only on P3, P10 and P30. There were significant increases in vertical movement and activity-chamber center time in the 0.4 xLD50 animals at P100 due to high variability, a supplementary cohort was exposed and tested no significant changes were found. These results suggest that the initial depression of AChE does not result in persistent changes that influence physiology or behavior.
- Chemical, Biological and Radiological Warfare