Proteomic Analysis of Genistein Mammary Cancer Chemoprevention
Annual rept. 1 Jul 2005-30 Jun 2006
ALABAMA UNIV IN BIRMINGHAM
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We have developed reproducible methodology for separation, biochemical and statistical analysis and identification of mammary gland proteins. We have discovered that GTP-CHI expression is significantly increased shortly following exposure at day 21 by genistein. At day 50, there was significant up-regulation of tyrosine hydroxylase and VEGF-R2. This and previous work suggests that early postnatal prepubertal exposure to genistein enhances cell proliferation and cell differentiation and gland maturation. This unique developmental maturation leads to a new biochemical blue-print whereby the cells have reduced EGF-signaling and VEGFR2 that render the mature mammary gland less proliferative and susceptible to chemically-induced mammary cancer initiation, angiogenesis and for cancer progression. Our experiments in identifying protein biomarkers in interstitial fluid surrounding mammary glands in rats have been mucli more diEcult and not successful to-date. We have recently developed our own probes that should allow us to collect a higher yield of proteins and hopefully allow success.
- Medicine and Medical Research