Prostate Cell Specific Regulation of Androgen Receptor Phosphorylation In Vivo
Annual rept. 30 Oot 2005-29 Oct 2006
NEW YORK UNIV SCHOOL OF MEDICINE NY
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On the basis of previous studies in which we analyze AR phosphorylation in vivo we propose that AR phosphorylation at serines 213 and 650 regulate differential target gene expression and recruitment to gene promoters via altered interaction with other cellular transcription factors. To test this hypothesis we have conducted yeast two-hybrid analysis with the N-terminus of wild type AR as well as AR S213A and AR S213E variants. Our preliminary analysis indicates that the screen is preferentially isolating proteins with a known role in gene transcription and we are currently assessing the phosphorylation- dependence of the putative AR interacting proteins.
- Anatomy and Physiology
- Medicine and Medical Research