Sam68 and Breast Cancer
Annual rept. 1 Aug 2005-31 Jul 2006
WAYNE STATE UNIV DETROIT MI
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Sam68 is a target for Src kinase. It is involved in HIV-1 RNA export. However, the role of Sam68 in cellular mRNA export is unknown. It has been well documented that patients have either lost or have mutations in BRCA1 or BRCA2 tumor suppressor genes are at high risk of developing breast cancers. Thus, the goal of this application is to investigate whether Sam68 is involved in the export of BRCA1 mRNA. To test this objective, we have generated stable Sam68 knockdown MCF7 cells. These reagents are critical to objectively test the role of Sam68 on BRCA1 expression. In this no-cost extension year, using MCF7 Sam68-RNAi stable cells, we expect to be able to examine how the reduced expression of Sam68 affects BRCA1 mRNA export. We will also identify the RNA export pathway for BRCA1 mRNA, proposed in Aim 2.
- Medicine and Medical Research