Three Dimensional Immobilization of Beta-Galactosidase on a Silicon Surface (Preprint)
GEORGIA INST OF TECH ATLANTA SCHOOL OF CIVIL ENGINEERING
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Many alternative strategies to immobilize and stabilize enzymes have been investigated in recent years for applications in biosensors. The entrapment of enzymes within silica-based nanospheres formed through silicification reactions provides high loading capacities for enzyme immobilization, resulting in high volumetric activity and enhanced mechanical stability. Here we report a strategy for chemically-associating silica nanospheres containing entrapped enzyme to a silicon support. Beta-Galactosidase was used as a model enzyme due to its versatility as a biosensor for lactose. The immobilization strategy resulted in a three-dimensional network of silica attached directly at the silicon surface, providing a significant increase in surface area and a corresponding 3.5-fold increase in enzyme loading compared to enzyme attached directly at the surface. The immobilized beta-galactosidase prepared by silica deposition was stable and retained more than 80 of its initial activity after 10 days at 24 degrees C. The ability to generate three-dimensional structures with enhanced loading capacity for biosensing molecules offers the potential to substantially amplify biosensor sensitivity.
- Inorganic Chemistry