Accession Number:

ADA464813

Title:

An Electrochemiluminescence-Based Competitive Displacement Immunossay for the Type-2 Brevetoxius in Oyster Extracts

Descriptive Note:

Journal article

Corporate Author:

ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FORT DETRICK MD

Report Date:

2006-07-24

Pagination or Media Count:

7.0

Abstract:

A new competitive electrochemiluminescence-based immunoassay for the type-2 brevetoxins in oyster extracts was developed. The assay was verified by spiking known amounts of PbTx-3 into 80 methanol extracts of Gulf Coast oysters. We also provide preliminary data demonstrating that 100 acetone extracts, aqueous homogenates, and the clinical matrixes urine and serum can also be analyzed without significant matrix interferences. The assay offers the advantages of speed 2 h analysis time simplicity only 2 additions, one incubation period, and no wash steps before analysis low limit of quantitation conservatively, 50 pgmL 1 ngg tissue equivalents and a stable, nonradioactive label. Due to the variety of brevetoxin metabolites present and the lack of certified reference standards for liquid chromatography-mass spectrometry confirmation, a true validation of brevetoxins in shellfish extracts is not possible at this time. However, our assay correlated well with another brevetoxin immunoassay currently in use in the United States. We believe this assay could be useful as a regulatory screening tool and could support pharmacokinetic studies in animals and clinical evaluation of neurotoxic shellfish poisoning victims.

Subject Categories:

  • Biology
  • Toxicology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE