Biosensor for Continuous Monitoring of Organophosphate Aerosols (Preprint)
APPLIED RESEARCH ASSOCIATES INC TYNDALL AFB FL
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An enzyme-based monitoring system provides the basis for continuous sampling of organophosphate contamination in air. The enzymes butyrylcholinesterase BuChE and organophosphate hydrolase OPH are stabilized by encapsulation in biomimetic silica nanoparticles, entrained within a packed bed column. The resulting immobilized enzyme reactors IMERS were integrated with an impinger-based aerosol sampling system for collection of chemical contaminants in air. The sampling system was operated continuously and organophosphate detection was performed in real-time by single wavelength analysis of enzyme hydrolysis products. The resulting sensor system detects organophosphates based on either enzyme inhibition of BuChE or substrate hydrolysis by OPH. The system proved suitable for detection of a range or organophosphates including paraoxon, demeton-S and malathion. The detection limits of the IMERs for specific organophosphates are presented and discussed.
- Agricultural Chemistry
- Organic Chemistry
- Physical Chemistry