Knockout AR in Prostate
Annual rept. 1 Oct 2005-30 Sep 2006
ROCHESTER UNIV NY
Pagination or Media Count:
Prostate cancer progresses from androgen-dependent to androgen-independent state. The androgen receptor AR is expressed throughout progression. We would like to understand the AR role in this progression. Using lox-Ore methodology we have generated mice in which AR function is abolished in the entire animal ARKO or tissue specific manner and generated mice with ARKO in prostate only or in different stages to be used to study prostate cancer PCa progresses. We aim to generate mice lacking AR in prostate epithelium. generate inducible ARKO mice to determine potential effects of androgen in AR absence on prostate growthmaintenance determine AR role in PCA developmentprogression by crossing ARKO mice with TRAMP mice to examine AR role in TRAMP induced PCa and permit determination of stages in PCa requiring AR function determine AR role in tumorigenicity of androgen- dependentandrogen-independent ARKO PCa cell lines. The effect of AR loss in these cells will be examined for ability to generatepromote tumors in mice. This year we generated mice with ARKO in the prostate epithelium and will be able to continue the other aims in the proposal in the coming years.
- Anatomy and Physiology
- Medicine and Medical Research