A Mouse Model to Investigate the Role of DBC2 in Breast Cancer
Annual summary rept. 15 Feb 2004-14 Feb 2007
TEXAS UNIV HEALTH SCIENCE CENTER AT SAN ANTONIO
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Sporadic breast cancer represents 90 of breast cancer patients. Mutations of both oncogenes and tumor suppressor genes often occur in spontaneous breast cancer. Specifically, tumor suppressor gene activity may be abrogated or decreased in cancer cells. Recently, a putative tumor suppressor gene, DBC2 Deleted in Breast Cancer, was discovered that appears to be frequently mutated in sporadic breast cancer. DBC2 is suspected to be a tumor suppressor gene important for breast cancer because 1 DBC2 expression cannot be detected in half of the spontaneous breast cancer tissues and cells tested and 2 wild-type WT DBC2 expressed in a breast cancer cell line, T47D, inhibited cellular proliferation while mutated DBC2 expression did not repress growth of the breast cancer cells. These data imply that mutation of DBC2 is important for the development of spontaneous breast cancer. This work serves ton investigate the functional role of DBC2 in cells and mice to elucidate the function of DBC2 for tumor suppression. To this end, we have begun targeting the DBC2 allele and are constructing vectors that will express wildtype human DBC2 cDNA and altered cDNAs.
- Genetic Engineering and Molecular Biology
- Anatomy and Physiology