Role of PAK6 in Prostate Cancer
Annual summary rept. 15 Sep 2005-14 Sep 2006
BETH ISRAEL DEACONESS MEDICAL CENTER BOSTON MA
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PAK6 is a serine threonine kinase whose expression is increased in prostate cancer.We have tried to understand the role played by PAK6 in PCa by finding its interacting partners.We have developed a strategy to find its interacting partners by tagging the protein with triple FLAG epitope,immunoprecipitating the protein using FLAG beads,elution by using triple FLAG peptide,running the eluted material on the gel,silver staining the gel and then mass spectrometry analysis is done on the specific bands.By this approach we have found nucleolin to be PAK6 interacting protein.Nucleolin is involved in cellular proliferation and it has also a role to play in cancer.Our another finding has shown that PAK6 is activated by MKK6 and p38 MAP kinase,so it implies PAK6 has some role to play under stress conditions.Nucleolin is also phosphorylated by p38 and its RNA binding ability increases under genotoxic stress depicting link between two findings.Another important potential partners of PAK6 found by this strategy are IQGAP1 and PP2C.
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