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Assessing the Effects of Soil Humic and Fulvic Acids on Germination and Early Growth of Native and Introduced Grass Varieties

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Interim rept. no. 2, 22 Sep-22 Mar 2006

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Experimental Five humic acid HA samples isolated from the two original collected in the field Wyoming soils in duplicate, Guernsey North GN45 and GN6 and Guernsey South GS45 and GS6, and one Utah soil, Dugway D11121314, and seven HA samples isolated from the same soils but collected in duplicate or triplicate from control no plants grown greenhouse pots GN-A and GN-B, GSA and GS-B, D-A, D-B and D-C, object of this research, were obtained from the USDA-St.Paul group. A solution 0.5 M NaOH was used to extract these HAs from soils. All HA samples were characterized for moisture and ash contents, elemental C, H, N, S, O and acidic functional group total acidity, COOH, phenolic OH composition, and by Fourier transform infrared FTIR spectroscopy, and fluorescence spectroscopy in the emission, excitation and synchronous scan modes. RESEARCH PLANS FOR THE REMAINDER OF THE CONTRACT PERIOD For the remainder of the contract period 14 months research plans are the following a Chemical and spectroscopic characterization of the HAs isolated in replicates from greenhouse pot soils where the two grassfour varieties were grown. b Germination and early growth experiments of the four grass varieties in combinations by two in the presence of the three soil HAs at two concentrations. c Possible follow-up experiments with HA concentrations optimal to promote the growth of the grass varieties of interest. d Correlation of the germination and seedling growth data with the chemical and spectroscopic parameters of the HAs examined, in order to possibly find out which HA parameters may influence germination and growth of the four grass varieties examined.

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  • Biology
  • Organic Chemistry
  • Numerical Mathematics

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