Prion Transport to Secondary Lymphoreticular System Tissues
Annual rept. 1 Jun 2005-31 May 2006
CREIGHTON UNIV OMAHA NE
Pagination or Media Count:
The long-term objective of this proposal is to identify mechanisms of prion transport to secondary lymphoreticular system LRS tissues. The hypothesis to be tested is that following peripheral exposure to prions host proteins e.g. complement bind prions allowing for trapping by cells in the spleen and enhancing uptake by macrophages, which are cells that are responsible for destruction of foreign proteins. To investigate this hypothesis we will examine the disease development of a prion strain DY TME that does not replicate in the spleen of hamsters. We will use this system to provide details into the host factors involved in transport of prions to cells in the LRS, such as spleen. We have shown differences in the susceptibility of HY and DY TME to phagocytosis and degradation by primary adherent peritoneal cells. We have shown differences in the spatial and temporal spread of the HY and DY TME agent in LRS tissues following intraperitoneal inoculation. We are currently investigating what cell types associate with these agents following inoculation and the proportion of each agent that is degraded.
- Anatomy and Physiology
- Medicine and Medical Research