Unique Proteins Expressed by Blood Vessels in Skeletal Sites Colonized by Breast Cancer Cells
Final rept. 1 Aug 2003-31 Jul 2006
PENNSYLVANIA STATE UNIV UNIVERSITY PARK
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This study focuses on identifying differences in the vasculature in the ends of long bones where breast cancer cells tend to lodge, as compared to the vasculature of the central marrow cavity. We developed a method to isolate highly pure populations of both cell types. We found differences in mRNA using microarray analysis and confirmed the data by RT-PCR. The bone-derived cells express five messages in greater abundance P2-fold or more than the marrow-derived cellsR conversely, the marrow-derived cells express higher levels of two other mRNAs. Possible roles may be fostering angiogenesis and cell survival. Using immunocytochemistry, we also found that the bone-derived cells present more of a cell surface adhesive protein, E-selectin. Taken together, the data show that the bone and marrow vasculatures are notably different in ways that could foster tumor growth within the bone compartment. At least one difference, surface presentation of E-selectin, is likely to be a factor in the specificity breast cancer cells have for bone environment.
- Anatomy and Physiology
- Medicine and Medical Research