Accession Number:

ADA461014

Title:

DNA Conforming Dynamics and Protein Binding

Descriptive Note:

Technical rept. 15 Aug 2005-14 Aug 2006

Corporate Author:

TEXAS UNIV AT BROWNSVILLE AND TEXAS SOUTHMOST COLLEGE

Personal Author(s):

Report Date:

2006-12-01

Pagination or Media Count:

8.0

Abstract:

Recent advances in singlemolecule force spectroscopy of DNA make it possible to study the thermodynamics and kinetics of DNA binding proteins under a wide range of conditions. A biophysical model for the DNA binding T4 gene 32 protein has been developed to study the kinetics of DNA protein binding to transient single-stranded DNA regions due to thermal fluctuations. The model is used to analyze recent single-molecule spectroscopy data of this system.

Subject Categories:

  • Genetic Engineering and Molecular Biology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE