A Rapid and Inexpensive PCR-Based STR Genotyping Method for Identifying Forensic Specimens
FEDERAL AVIATION ADMINISTRATION OKLAHOMA CITY OK CIVIL AEROMEDICAL INST
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Situations arise where forensic samples can be inadvertently misidentified during field collection. Samples identified in the field as being from the same person that return conflicting toxicological results may suggest such misidentification. Polymerase chain reaction PCR-based human identity testing provides a reliable and independent method to confirm sample identification. In the study presented here, the Federal Bureau of Investigations human Combined DNA Identity System CODIS loci were used on a rapid, inexpensive microfluidics chip electrophoresis platform to confirm the identity of forensic samples from an aircraft accident site. Absolute allele identification was not achieved with this method, but it was found to be suitable for comparative analysis, as demonstrated by validation of the results and conclusions from capillary electrophoresis. The nine CODIS Short Tandem Repeat loci and a gender discrimination locus used in this study have a greater than 5E07 matching probability suitable for small sample sizes.
- Medicine and Medical Research
- Test Facilities, Equipment and Methods
- Fluid Mechanics